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Not Data Review. Releview.

When you think about data review for PCR data, what’s the first thing that comes to mind? Traces – lots and lots of traces. It is not uncommon to sift through literally hundreds of amplification curves, each day. Why have you been having to manually wade through all these results? Why can’t you just see what’s relevant to getting the batch reviewed, certified, and reported?

Reduce the time and effort of reviewing and increase your confidence in the final report.

The reason you’ve been looking at all those amplification curves is because, in some way, you can’t be sure that the curves which the data system is presenting are, in fact, reflective of the true amplification. If you can’t trust the traces, you can’t trust the values derived from the traces (such as Ct value). Data systems have had to include ways with which to visualize this mass of amplification curves – and all of the calculated values.

ARQ changes this in a way that reduces the time and effort necessary to perform review while providing even more confidence about the quality of your results. The concept is called Releview: the idea that an enormous acceleration of the normal data review process is possible by focusing on what’s relevant at exactly your step of the review process. Starting with ARQ’s curve processor itself and building on the customized quality rule system, Indigo has reimagined the manner in which review can be done. Rather than starting with the notion that every single trace has to be reviewed, ARQ begins with the notion that only those results which represent an exception of some type truly need to be manually reviewed. Reviewing those exceptions is done from a different viewpoint by different users – each of them searching for what is relevant to their own perspective.

Driven by your quality rules to help you more accurately assess curves.

Exceptions can be actionable or informative, and are driven by a set of quality rules which are defined by you and automatically run by the system. ARQ’s curve processor, as powerful as it is, may still run into instances where manual inspection is required. ARQ notifies you of those traces which should be reviewed, and presents them with a set of sigmoid-focused flags. Once the curves have been reviewed, other areas of the result set can also be considered: how are the QC samples performing, is there any indication of cross well contamination, are any sample observations outside of certain ranges?

Using both automation and human review to help your team make more informed decisions.

ARQ’s batch review pages allow you to select specific categories of quality rules and exceptions, prioritizing and visualizing the results which correspond to your needs. Releview combines the computing power of automation with the human power of exception based review, to deliver certified results as fast as possible. This is a fundamentally better way to work through the wide array of data and values which make up the results. Treating these processes as a team of the reviewer and ARQ working together provides a more holistic view of the workflow, resulting in the team being able to make more informed decisions in less time.

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